Errors and Error Rate versus Position. Unpublished results in a traditional SBL sequencing run. These reads are separated into two parts, A and B, and are designed either M or P for Minus or Plus, away or towards the site of attachment on the bead. These AM and AP reads are obtained using different hybridized primers. Error and error rates, when not using a cyclic or digestion method, results in loss of specificity the further away a base is from the site of ligation.