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Table 1 Sequences of the Test Template, various Anchor Primers, and Query Primers.

From: Sequencing by ligation variation with endonuclease V digestion and deoxyinosine-containing query oligonucleotides

Sequence Names DNA Sequence
Test Template 5' (Dual Biotin) TCT ATG GGC AGT CGG TGA T AN GCG CTT GCA AGA GAA TGA GGA AAA CGA AGA 3'
Anchor Primer 5' (Phosphate) A TCA CCG ACT GCC CAT AGA 3'
-1 Anchor Primer 5' (Phosphate) TCA CCG ACT GCC CAT AGA 3'
-2 Anchor Primer 5' (Phosphate) CA CCG ACT GCC CAT AGA 3'
-3 Anchor Primer 5' (Phosphate) A CCG ACT GCC CAT AGA 3'
ExSeq4 - A 5' Cy3 - NNINNANNN 3'
ExSeq4 - T 5' TYE 665 (Cy5 Analog)- NNINNTNNN 3'
ExSeq4 - C 5' 6-FAM (FITC Analog)- NNINNCNNN 3'
ExSeq4 - G 5' TEX 615 (Texas Red Analog)- NNINNGNNN 3'
Saturation Primer 5' NNINNNNNN 3'
  1. Deoxyinosine is indicated by "I." Degenerate bases represented by "N." Underlined bases are areas of anchor primer hybridization.