Figure 6

Mean expression levels within the Terpenoid Backbone, Diterpenoid and Zeatin Biosynthesis pathways. A) Normalized mean expression levels for enzymes within the Terpenoid Backbone Biosynthesis (TBB) pathways, namely, plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, Diterpenoid Biosynthesis (DB) pathway and Zeatin Biosynthesis (ZB) pathway are provided. B) Normalized mean expression levels for enzymes within the TBB pathways, namely, cytosolic mevalonic acid (MVA) pathway, DB and ZB pathways. Number of E. fischeriana transcripts, from distinct gene clusters, matching each enzyme are shown between brackets in panels A and B. Abbreviations: AACT, acetoacetyl-coenzyme A (CoA) thiloase; CMS, 2-C-methyl-erythritol 4-phosphate cytidyl transferase; DXR, 1-deoxy-D-xylulose 5-phosphate reductoisomerase; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FPPS, farnesyl diphosphate synthase; GGPPS, geranylgeranyl diphosphate synthase; GPPS, geranyl diphosphate synthase; NA, Not Available; HMGR, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase; IPI, isopentenyl diphosphate isomerase; MK, mevalonate kinase; MPK, mevalonate-5-phosphate kinase; CMK, 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase; MDD, mevalonate diphosphate decarboxylase; IDS, isopentenyl diphosphate/dimethylallyl diphosphate synthase; MCS, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; HDS, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase; HMGS, HMG-CoA synthase; HMG-CoA, 3S-hydroxy-3-methylglutaryl coenzyme A; DXP, 1-deoxy-D-xylulose 5-phosphate; MVA, 3R-Mevalonic acid; M5P, Mevalonate-5-phosphate; MPP, Mevalonate diphosphate; MEP, 2-C-methyl-D-erythritol 4-phosphate; CDP-ME, 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol; CDP-MEP, 4-(cytidine 5'-diphospho)-2C-methyl-D-erythritol 2-phosphate; cMEPP, 2C-methyl-D-erythritol 2,4-cyclodiphosphate; DMAPP, Dimethylallyl diphosphate; HMBPP, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate; IPP, isopentenyl diphosphate; G3P, Glyceraldehyde 3-phosphate; GPP, geranyl diphosphate; GGPP, geranylgeranyl diphosphate; FPP, farnesyl diphosphate; GGPPS, geranylgeranyl diphosphate; ent-KSA, ent-Kaurene synthase A; ent-KSB, ent-Kaurene synthase B; ent-Kox, ent-Kaurene oxidase; CS, Casbene synthase; tRNA-DMAT, tRNA Dimethylallyltransferase; cis-ZOG, cis- Zeatin O-beta-D-glucosyltransferase; ent-CPP, ent-Copalyl diphosphate; ent-K, ent-Kaurene; UDP, Uridine 5'-diphosphate.