Figure 7

The O. fasciatus transcriptome adds sequence data to existing GenBank accessions, which in turn improves annotation of transcriptome sequences. (A) Extended contig for Of-hunchback (bottom), comprising the complete mRNA GenBank accession (top, light grey), two isotigs and one CAP3 contig from the transcriptome (middle, dark grey). The largest isotig provides an additional 252 bp of 3' UTR sequence to the GenBank sequence (black). Comparison with the GenBank sequence enabled isotig 08619 and cap3_contig 21314 to be assembled into the same contig. (B) Extended contig for Of-homothorax (bottom), with a partial mRNA GenBank accession (top, light grey) and two transcriptome isotigs (middle, dark grey). Both isotigs extend beyond the known GenBank sequence at the 3' and 5' ends, extending the known region by 449 bp in total (black). Both isotigs had been identified as homothorax, and because they did not overlap, they were classified as belonging to the same transcript rather than being paralogs. The GenBank sequence bridges an 87 bp gap between the isotigs, confirming that both sequences are fragments of a single gene.