Phylogenetic analysis of the Simulium collagen-like family. (A) Clustal alignment. (B) Bootstrapped phylogram of the alignment in (A). The Simulium guianense proteins are indicated by a square, the Simulium nigrimanum proteins by a circle, and the Simulium vittatum proteins by a triangle. The symbols above of the alignment indicate (*) identical sites, (:) conserved sites, and (.) less-conserved sites. The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The bar at the bottom represents 5% amino acid substitution. Protein sequences were aligned by the Clustal program, and the dendogram was made with the Mega package after 10 000 bootstraps with the neighbor-joining algorithm. For other details, see Figure 3.