Figure 3

The amylase/maltase family in Diptera. Phylogram derived from the alignment of two Simulium guianense proteins (indicated by a square and starting with "Sg-"), with their best matches in the non-redundant protein database of the National Center for Biotechnology Information identified by the three first letters of the genus name, followed by the three first letters of their species name, followed by their NCBI accession numbers. Glycosidases from Simulium vittatum and Simulium nigrimanum are indicated by a triangle and a circle, respectively. The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The bar at the bottom represents 10% amino acid substitution. Protein sequences were aligned by the Clustal program, and the dendogram was made with the Mega package after 10 000 bootstraps with the neighbor-joining algorithm.