Phylogeny of the salivary serine protease family of S. Guianense. Phylogram derived from the alignment of three Simulium guianense proteins and their best matches from the non-redundant protein database from the National Center for Biotechnology Information. S. guianense proteins are indicated by a square and start with Sg-. Serine proteases from Simulium nigrimanum (SIMNIG) are indicated by a circle and in Simulium vittatum (SIMVIT) by a triangle, with their respective accession numbers. The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The bar at the bottom represents 20% amino acid substitution. Protein sequences were aligned by the Clustal program, and the dendogram was made with the Mega package after 10 000 bootstraps with the neighbor-joining algorithm. For other details, see Figure 3.