Phylogram of two Antigen-5 proteins derived from Simulium guianense sialotranscriptome with their best matches from the non-redundant protein database from the National Center for Biotechnology Information. The S. guianense proteins (Sg-457 and Sg-453) are indicates by a square, Simulium vittatum proteins by a triangle, and S. nigrimanum proteins by a circle. The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The bar at the bottom represents 10% amino acid substitution. Protein sequences were aligned by the Clustal program, and the dendogram was made with the Mega package after 10 000 bootstraps with the neighbor-joining algorithm. For other details, see Figure 3.