The Simulium vittatum erythema protein (SVEP) superfamily of Simulium. (A) Clustal alignment of all SVEP proteins derived from black fly sialotranscriptomes. The symbols above the alignment indicate (*) identical sites, (:) conserved sites, and (.) less-conserved sites. (B) Bootstrapped phylogram derived from the alignment in (A). Simulium guianense proteins are indicated by a square, S. vittatum by a triangle, and Simulium nigrimanum by a circle. The numbers on the tree bifurcations indicate the percentage bootstrap support above 75%. The bar at the bottom represents 10% amino acid substitution. Protein sequences were aligned by the Clustal program, and the dendogram was made with the Mega package after 10 000 bootstraps with the neighbor-joining algorithm. For other details, see Figure 3.