Mapping of the ChrUns 004.35 and 004.39. The complex mapping on UMD of sequences composing the Btau unassigned scaffolds 004.35 and 004.39 is illustrated by a Circos graphic output in (a) and (d), respectively. The red ovals indicate the bioinformatics mapping of the BACs used in the FISH experiments, results of which are reported in the boxes below. The half-oval indicates that the mapping of the BAC U35a was inferred by the matching of a single BES. The second BES was found on chr2 in both drafts. (b) FISH results of BAC U35a confirming its unique localization on chr2. (c) Multiple signals yielded by BAC U35b (see Circos graphics in 5a). (e) FISH results of BAC U39a (red) cohybridized with chr15- and chr23-reference BACs (green and blue spots, respectively) showing that it maps on chr23 and not on chr15. (f) Multiple FISH signals produced by BAC U39b (see text for details).