Table 2 qRT-PCR validation of RNA-seq data on a random selection of thirteen genes

AAEL006138-RA

Vitellogenin-A1 (3)

118666

8

12.82

2.67 (0.44)

9.68^-4 (7.84^-4)

11.43**

AAEL013284-RA

Serine-type endopeptidase AaLT (27)

22558

19

9.18

0.97 (0.61)

1.54^-2 (2.51^-2)

5.98*

AAEL013707-RA

Trypsin-1 (28)

7612

32

6.86

1.28 (0.23)

6.96^-2 (3.50^-2)

4.20**

AAEL001806-RA

Lipid binding (2)

171

1

6.38

0.19 (2.63^-2)

3.39^-2 (1.04^-2)

2.50**

AAEL014734-RA

Catalytic activity (0)

7770

802

2.24

3.08^-2 (2.63^-2)

7.65^-3 (7.89^-3)

2.01

AAEL011470-RA

Protein binding (0)

4551

615

1.85

5.91^-2 (2.39^-2)

3.98^-2 (1.27^-2)

0.57

AAEL013005-RA

Molecular function (0)

1833

253

1.82

1.15^-2 (3.55^-3)

7.21^-3 (3.41^-3)

0.67

AAEL002565-RA

Structural constituent of Cytoskeleton (16)

15421

21389

-1.51

6.52^-2 (1.66^-2)

9.13^-2 (1.32^-2)

-3.81*

AAEL008848-RA

Catalytic activity (0)

9414

11236

-1.29

0.30 (0.13)

0.62 (0.13)

-1.05*

AAEL012175-RA

Catalytic activity (4)

36942

41536

-1.21

0.35 (0.08)

1.42 (1.23)

-2.02

AAEL011871-RA

Electron Transporter (0)

11042

12116

-1.17

0.70 (0.40)

2.27 (0.42)

-1.70**

AAEL006425-RA

Serine-type endopeptidase (28)

1403

13914

-4.34

0.17 (4.62^-2)

1.52 (0.39)

-3.16**

AAEL008701-RA

Iron Ion Binding (0)

51

689

-4.79

2.60^-3 (7.57^-4)

0.196 (3.92^-2)

-6.24**