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Table 1 RT-PCR validation of selected genes from the microarray

From: Differential gene expression in liver and small intestine from lactating rats compared to age-matched virgin controls detects increased mRNA of cholesterol biosynthetic genes

RT-PCR
Measurements
Transcript
Cluster ID
RL RD RJ RIL
Tmem97 7080099 1.52
(p = 0.021)
2.60
(p < 0.01)
1.59
(p = 0.42)
1.73
(p = 0.072)
Npc1l1 - 1.00
(p = 1.00)
1.27
(p = 0.49)
1.22
(p = 0.16)
0.88
(p = 0.72)
Abcb1a 7250393 0.82
(p = 0.91)
0.83
(p = 0.77)
0.72
(p = 0.09)
0.65
(p < 0.01)
Cyp1a1 7336681 2.98
(p = 0.93)
1.12
(p = 0.64)
2.06
(p < 0.01)
0.78
(p = 0.89)
Cyp3a23/3a1 7100149 1.83
(p = 0.12)
1.30
(p = 1.00)
BDL BDL
Fdft1 7139070 1.36
(p = 0.11)
2.58
(p = 0.22)
1.02
(p = 0.99)
2.41
(p = 0.018)
Hmgcr 7202670 2.05
(p < 0.01)
1.52
(p = 0.30)
1.14
(p = 0.80)
1.18
(p = 0.48)
Slc39a4 7329323 2.71
(p = 0.59)
2.92
(p < 0.01)
2.20
(p < 0.01)
1.99
(p < 0.01)
Sqle 7317317 1.80
(p < 0.01)
2.66
(p = 0.034)
1.71
(p = 0.50)
1.59
(p = 0.056)
Ugt2b36 (Ugt2b4) 7117373 0.85
(p = 0.047)
0.76
(=0.41)
0.39
(p = 0.21)
0.62
(p = 1.00)
Microarray
Mean Log2
Measurements
  RL RD RJ RIL
Tmem97 7080099 1.92
(p < 0.01)
2.03
(p < 0.01)
1.82
(p < 0.01)
2.15
(p < 0.01)
Abcb1a 7250393 0.63
(p < 0.01)
0.66
(p < 0.01)
0.65
(p < 0.01)
0.70
(p = 0.01)
Cyp1a1 7336681 2.29
(p < 0.01)
1.23
(p = 0.35)
1.71
(p = 0.02)
0.67
(p = 0.03)
Cyp3a23/3a1 7100149 1.69
(p < 0.01)
0.98
(p = 0.81)
1.05
(p = 0.50)
1.04
(p = 0.58)
Fdft1 7139070 1.38
(p < 0.01)
1.98
(p < 0.01)
1.42
(p < 0.01)
1.55
(p < 0.01)
Hmgcr 7202670 1.97
(p < 0.01)
1.64
(p < 0.01)
1.17
(p = 0.14)
1.38
(p < 0.01)
Slc39a4 7329323 2.81
(p < 0.01)
1.89
(p < 0.01)
1.65
(p < 0.01)
1.68
(p < 0.01)
Sqle 7317317 1.83
(p < 0.01)
2.27
(p < 0.01)
1.72
(p < 0.01)
1.70
(p < 0.01)
Ugt2b36 (Ugt2b4) 7117373 0.96
(p = 0.86)
0.57
(p < 0.01)
0.37
(p < 0.01)
1.00
(p = 0.97)
  1. Ratios were calculated as Lactating measurement divided by Control measurement. RT-PCR data is reported as the ratio of normalized measurements, and microarray data is reported as the ratio of normalized, untransformed intensities. p-Values were calculated using a mixed models approach as described in methods. RL, RD, RJ and RIL are the Ratio of Lactation to Control in liver (L), duodenum (D), jejunum (J), and ileum (IL), respectively. BDL, below detection limit