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Table 1 RT-PCR validation of selected genes from the microarray

From: Differential gene expression in liver and small intestine from lactating rats compared to age-matched virgin controls detects increased mRNA of cholesterol biosynthetic genes

RT-PCR

Measurements

Transcript

Cluster ID

RL

RD

RJ

RIL

Tmem97

7080099

1.52

(p = 0.021)

2.60

(p < 0.01)

1.59

(p = 0.42)

1.73

(p = 0.072)

Npc1l1

-

1.00

(p = 1.00)

1.27

(p = 0.49)

1.22

(p = 0.16)

0.88

(p = 0.72)

Abcb1a

7250393

0.82

(p = 0.91)

0.83

(p = 0.77)

0.72

(p = 0.09)

0.65

(p < 0.01)

Cyp1a1

7336681

2.98

(p = 0.93)

1.12

(p = 0.64)

2.06

(p < 0.01)

0.78

(p = 0.89)

Cyp3a23/3a1

7100149

1.83

(p = 0.12)

1.30

(p = 1.00)

BDL

BDL

Fdft1

7139070

1.36

(p = 0.11)

2.58

(p = 0.22)

1.02

(p = 0.99)

2.41

(p = 0.018)

Hmgcr

7202670

2.05

(p < 0.01)

1.52

(p = 0.30)

1.14

(p = 0.80)

1.18

(p = 0.48)

Slc39a4

7329323

2.71

(p = 0.59)

2.92

(p < 0.01)

2.20

(p < 0.01)

1.99

(p < 0.01)

Sqle

7317317

1.80

(p < 0.01)

2.66

(p = 0.034)

1.71

(p = 0.50)

1.59

(p = 0.056)

Ugt2b36 (Ugt2b4)

7117373

0.85

(p = 0.047)

0.76

(=0.41)

0.39

(p = 0.21)

0.62

(p = 1.00)

Microarray

Mean Log2

Measurements

 

RL

RD

RJ

RIL

Tmem97

7080099

1.92

(p < 0.01)

2.03

(p < 0.01)

1.82

(p < 0.01)

2.15

(p < 0.01)

Abcb1a

7250393

0.63

(p < 0.01)

0.66

(p < 0.01)

0.65

(p < 0.01)

0.70

(p = 0.01)

Cyp1a1

7336681

2.29

(p < 0.01)

1.23

(p = 0.35)

1.71

(p = 0.02)

0.67

(p = 0.03)

Cyp3a23/3a1

7100149

1.69

(p < 0.01)

0.98

(p = 0.81)

1.05

(p = 0.50)

1.04

(p = 0.58)

Fdft1

7139070

1.38

(p < 0.01)

1.98

(p < 0.01)

1.42

(p < 0.01)

1.55

(p < 0.01)

Hmgcr

7202670

1.97

(p < 0.01)

1.64

(p < 0.01)

1.17

(p = 0.14)

1.38

(p < 0.01)

Slc39a4

7329323

2.81

(p < 0.01)

1.89

(p < 0.01)

1.65

(p < 0.01)

1.68

(p < 0.01)

Sqle

7317317

1.83

(p < 0.01)

2.27

(p < 0.01)

1.72

(p < 0.01)

1.70

(p < 0.01)

Ugt2b36 (Ugt2b4)

7117373

0.96

(p = 0.86)

0.57

(p < 0.01)

0.37

(p < 0.01)

1.00

(p = 0.97)

  1. Ratios were calculated as Lactating measurement divided by Control measurement. RT-PCR data is reported as the ratio of normalized measurements, and microarray data is reported as the ratio of normalized, untransformed intensities. p-Values were calculated using a mixed models approach as described in methods. RL, RD, RJ and RIL are the Ratio of Lactation to Control in liver (L), duodenum (D), jejunum (J), and ileum (IL), respectively. BDL, below detection limit