Figure 2

N-terminal signal peptides detected in Shewanella. Note that some T5SS substrates have been reported to possess an additional n- and h- region at the N-terminus and thus the position of the signal peptidases cleavage site would likely go undetected by standard predictors such as SignalP. Arrows indicate the position at which the signal peptide is cleaved by the respective signal peptidases. Conserved sequences are indicated, with X denoting any amino acid. The twin arginine motif is denoted by ZXRRXϕϕ, where Z=hydrophilic residue and ϕ=hydrophobic residue.