Figure 2

Genetic complementation results for MA4265. Growth of isogenic E. coli strains in the BW25113 (WT) background carrying deletion mutant alleles icd (a), leuB (b), and yeaU (c), and transformed with an empty control vector (EV=pET-21a) or a plasmid expressing MA4265. All strains were grown on M9 minimal media with glucose (or malate in plate c) as the carbon source, and contained 100 µg/mL ampicillin. Plates were incubated at 37°C for 48 hr. For panel (c), D-malate was substituted for glucose as the sole carbon source to examine the growth phenotype of yeaU[23]. d) The icd mutant strain carrying either the empty control vector (icd+EV) or the MA4265-expressing vector (icd+MA4265) and the parental strain (WT) empty control vector (WT+EV) or the MA4265-expressing vector (WT+MA4265) were grown in M9 liquid media with glucose as the carbon source and 100 µg/mL ampicillin. Cultures were incubated at 37°C and OD₆₀₀ was monitored. Data from the average of three replicates (± standard error) are presented.