Accurate and fast estimation of taxonomic profiles from metagenomic shotgun sequences

BMC Genomics

Table 2 Simulated metagenomic sample

Species	Coverage	Abundance	Genome Size	# 16S rRNA
Bifidobacterium bifidum PRL2010	25	50%	2.2Mbp	3 copies
Bacteroides fragilis NCTC 9343	10	20%	5.1Mbp	6 copies
Staphylococcus aureus USA300	5	10%	2.8Mbp	5 copies
Enterococcus faecalis V583	5	10%	3.2Mbp	4 copies
Clostridium difficile 630	5	10%	4.2Mbp	11 copies

To evaluate the performance of different approaches in estimating the bacterial composition, we have created a simulated metagenomic sample consisting of 5 species with 100bp reads. ”Coverage” indicates the depth of the coverage of the simulated reads in the simulated sample for the genomes.

ISSN: 1471-2164