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Table 1 Statistics of small RNA sequences from Chlamydomonas reinhardtii + S and -S libraries

From: Characterization and differential expression of microRNAs elicited by sulfur deprivation in Chlamydomonas reinhardtii

  Sequences generated %
+S library   
Total reads 11284767  
High quality 10762852 100%
Adaptor3 null 3304 0.03%
Insert null 2815 0.03%
Adaptor5 contaminants 63608 0.59%
Smaller than 18 nt 663116 6.16%
PolyA 17 0.00%
Clean reads 10029992 93.19%
-S library   
Total reads 11080539  
High quality 10572756 100%
Adaptor3_null 3499 0.03%
Insert null 3575 0.03%
Adaptor5 contaminants 58280 0.55%
Smaller than 18 nt 588466 5.57%
PolyA 6 0.00%
Clean reads 9918931 93.82%
  1. Total reads: total sequenced reads; High quality: number of high quality reads with no N, no more than 4 bases whose quality score is lower than 10 and no more than 6 bases whose quality is lower than 13; Adaptor3 null: number of reads with no 3' adaptor; Insert null: number of reads with no insertion; Adaptor5 contaminants: number of 5' contaminants; Smaller than 18 nt: number of reads less than 18 nt. Generally, small RNA tags are between 18 ~ 30 nt long. So too short tags should be removed from data for future analysis; polyA: number of reads wih polyA; Clean reads: number of clean reads after adaptors and contaminants are removed which are used in the following analysis.
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BMC Genomics

ISSN: 1471-2164

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