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Table 4 Predicted Chlamydomonas reinhardtii miRNAs that are responsive to sulfur-deprivation

From: Characterization and differential expression of microRNAs elicited by sulfur deprivation in Chlamydomonas reinhardtii

Name

Sequence (5'-3')

L (nt)

Location in the geonome

MFE (kcal mol-1)

Reads +S/-S

n006

UCCAGCUGGGCGGCCGUCUCC

21

chromosome_10:4733426:4733510:-

-38.6

1004/2798

n015

UUCUACCCAAGAGGCUGUGUA

21

chromosome_12:2479175:2479337:+

-93.9

84/264

n030

UCAAAGCUAGGAGCCAUGAAG

21

chromosome_14:139326:139506:+

-129.4

1347/4625

n046

UGUUCGGAGAUCCUUGUGCAUG

22

chromosome_16:3072001:3072107:-

-101.1

117/373

n051

UUGUUGACGACGUGCGCGGGC

21

chromosome_17:204043:204314:-

-129.8

41137/86593

n052

UGACACAUGGAACAACACAAC

21

chromosome_1:3554627:3554814:+

-150.9

287/505

n062

UGACAUGCGGUGAAUGUGAAU

21

chromosome_3:5564702:5564806:+

-105.4

15185/49088

n077

UCAUGAAGCGGAUACUGUGAA

21

chromosome_7:743887:744064:-

-103.91

1160/61

n083

UGGGCCUGUUGUGCACGUUCC

21

scaffold_28:208520:208678:-

-115.9

104/662

n084

UUGUGCCGGCCGACACUGCGG

21

scaffold_33:45408:45519:+

-49.9

77092/55878

n152

UGUACGGCGACCUGCAAAUGG

21

chromosome_10:5980485:5980652:+

-115.7

0/215

n168

AGGAUGACCGUCAUGAUUGCG

21

chromosome_13:4630466:4630659:-

-167

0/4628

n169

GUCAUUAAGACCGUCGGCAAU

21

chromosome_14:1815533:1815835:+

-206.26

0/170

n182

UAGGGCUUUUCGGAAGGGAGA

21

chromosome_17:4302900:4303054:+

-83.2

0/20463

n196

AUUCACAUUCACCGCAUGUCA

21

chromosome_3:5564693:5564816:+

-112

0/16257

n197

UCCUCCUCCUUGACGUCGGCG

21

chromosome_3:7309395:7309503:+

-50.1

0/321

n198

AGCAGCUUCCCACUCCCACGACC

23

chromosome_3:2104211:2104422:-

-66.5

0/415

n200

UGCGCAGCGGCAUCAUCUGGA

21

chromosome_4:2994696:2994894:-

-157.7

0/367

n207

UAGCAGUCUGAACCAAAGUCG

21

chromosome_7:2564607:2564734:+

-92.1

0/931

n209

UAUGGGCAGUUGUACUAAAUC

21

chromosome_7:4354369:4354571:+

-136.8

0/564

n212

UGGGCCUCACGGCGGCGGACC

21

chromosome_7:5449979:5450131:+

-140.8

0/270

n214

AGGGCCAACAGCUUUGACCGG

21

chromosome_7:5670376:5670487:+

-103.9

0/557

n222

AAUGCCAGCAGCUCCACGCCC

21

scaffold_18:257:618:+

-158.6

0/4384

  1. Name: the name of predicted Chlamydomonas reinhardtii miRNAs that are responsive to sulfur-deprivation; Sequence: sequence cloned in small RNA libraries; L, the length of miRNA; MFE: the adjusted minimum free energy (MFE) representing the MFE of 100 nucleotides; Reads: the effect of sulfur-deprivation on miRNA expression, +S/-S, normalized sequencing frequencies in the +S and -S library.