Figure 5
Examples of exons with splicing differences between placental and HBM2.0 tissues. (a) Exon ENSE00000882762 in ITGA6. (b) Exon ENSE00001385284 in ITGB4. (c) Exon ENSE00000736978 in TCIRG1. Shown on the left-hand side are wiggle plots of RNA-Seq read coverage and RT-PCR gel images for validation of differential splicing events generated for placental and HBM2.0 tissues. UJC, DJC, and SJC indicate upstream, downstream, and skipping junction counts, respectively. Star mark in (c) indicates an additional alternatively spliced product detected by using the given primer pairs. Represented on the right-hand side are histograms showing exon inclusion levels obtained from RNA-Seq (blue bar) and RT-PCR (red bar) experiments. The values represented by red bars correspond to the numbers shown on the top of the gel pictures.