Genetic analysis of the KG line. (A) Mating scheme. KG♀×KG♂ and KG♀×WT♂, but not WT♀×KG♂ generated masculinized females. Sib-crosses weakend masculinized phenotypes, but KG♀×WT♂ reactivated masculinization. (KG♀×WT♂)♀×WT♂ individuals were used in this study. See also Table 1. (B) The molecular structure of KG W chromosome. Genomic PCR analyses were performed by using eight W chromosome-specific RAPD markers . Arrowheads indicate W chromosome-specific amplicons.