Differential expression of cellular genes after induction of wild-type and mutant Tax proteins in HeLa cells. Total RNA from HeLa cells transfected with pCAGGS encoding Tax proteins (TaxWT, TaxS240P, or TaxD247G) or the control pCAGGS was used for the microarray analysis. (A) Heat map of hierarchical gene clustering. The microarray data was analyzed using GeneSpring GX 11.0 software to obtain the hierarchical clustering. The columns correspond to gene profiles regulated by the different Tax proteins indicated above the map. Below the heat map is a color-coded scale bar for the relative expression levels of genes. ( B) Number of genes significantly up- or downregulated by wild-type and mutant Tax proteins. The genes shown in this figure passed 2 filtering criteria: (1) statistical significance (p < 0.05) and (ii) at least 2-fold changes in the expression of a cellular gene at least 1 time point.