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Table 2 Genes selected for analysis with respect to cellulase expression and deletion strains

From: Unravelling the molecular basis for light modulated cellulase gene expression - the role of photoreceptors in Neurospora crassa

Strain

locus ID

description/function

topic/selection criteria

potential contribution to cellulolytic efficiency in

FGSC12595

NCU00365

hypothetical protein, repressed by CPC-1

amino acid metabolism

 

FGSC14540

NCU03935

homoserine dehydrogenase

amino acid metabolism

 

FGSC21460

NCU04050

cpc-1 encoding cross pathway control protein

amino acid metabolism

Δwc-1 and Δwc-2

FGSC17355

NCU04482

hypothetical protein, upregulated by aa starvation

amino acid metabolism

Δwc-1 and Δwc-2

FGSC19959

NCU06724

glutamine synthetase

amino acid metabolism

 

FGSC14944

NCU02500

ccg-4 alpha type peptide pheromone

consistent regulation

 

FGSC18933

NCU06687

glycogen synthase

consistent regulation/glycogen metabolism

 

FGSC20155

NCU07027

glycogen phosphorylase

consistent regulation/glycogen metabolism

 

FGSC17911

NCU08226

glycosyl transferase family 2

consistent regulation/glycogen metabolism

Δwc-2

FGSC11557

NCU00104

heat shock protein, HSP98-like

differential regulation by WC-1 and WC-2

 

FGSC17055

NCU00716

non-anchored cell wall protein, ncw-5

differential regulation by WC-1 and WC-2

Δvvd and Δwc-2

FGSC11568

NCU07232

heat shock protein 30, light responsive

differential regulation by WC-1 and WC-2

 

FGSC11202

NCU00355

catalase-3

fenton chemistry/oxidative depolymerization

Δvvd

FGSC16398

NCU00829

ferric reductase

fenton chemistry/oxidative depolymerization

Δvvd

FGSC13139

NCU01873

cellobiose dehydrogenase

fenton chemistry/oxidative depolymerization

 

FGSC16325

NCU02948

quinone oxidoreductase, type IV; light responsive; non-anchored cell wall protein, ncw-4

fenton chemistry/oxidative depolymerization

 

FGSC11223

NCU03013

copper/zinc super oxide dismutase (SOD); anchored cell wall protein, acw-10

fenton chemistry/oxidative depolymerization

 

FGSC11351

NCU05113

multicopper oxidase/laccase precursor

fenton chemistry/oxidative depolymerization

Δwc-2

FGSC13601

NCU05595

cellobiose dehydrogenase

fenton chemistry/oxidative depolymerization

 

FGSC13819

NCU05923

cellobiose dehydrogenase

fenton chemistry/oxidative depolymerization

 

FGSC20315

NCU08432

cellobiose dehydrogenase

fenton chemistry/oxidative depolymerization

 

FGSC10372

NCU08807

carbon catabolite repressor cre-1

target of the white collar complex

 

FGSC16190

NCU02240

endoglucanase II, glycosyl hydrolase family 61

up-regulation in Δvvd

 

FGSC16218

NCU02344

cellulose binding protein CEL1, glycoside hydrolase family 61

up-regulation in Δvvd

 

FGSC16318

NCU02663

L-lysine 2,3 aminomutase; radical SAM superfamily

up-regulation in Δvvd

 

FGSC16228

NCU02855

endo 1,2 beta xylanase A; glycosyl hydrolase family 11

up-regulation in Δvvd

Δvvd

FGSC16379

NCU03753

grg-1 encoding glucose repressible protein; clock controlled protein CCG-1

up-regulation in Δvvd

 

FGSC13439

NCU05159

acetylxylan esterase, contains fungal cellulose binding domain

up-regulation in Δvvd

 

FGSC18946

NCU07787

clock controlled gene ccg-14, probable Snodprot1, ceratoplatanin like

up-regulation in Δvvd

 

FGSC19600

NCU07898

endoglucanase IV, glycosyl hydrolase family 61

up-regulation in Δvvd

 

FGSC18480

NCU10045

pectin esterase

up-regulation in Δvvd

 

FGSC13753

NCU08192

fungal hydrophobin, magnaporin related

up-regulation in Δwc-1 and Δwc-2

 

FGSC13811

NCU05789

endo 1,3 (4) beta glucanase, glycosyl hydrolase family 16

up-regulation in Δwc-2

 
  1. Strains are ordered according to the criteria for which they were selected for analysis. Selection criteria correspond to the topics important for evaluation of cellulase production in the respective mutant. The result of this evaluation was used to determine whether this gene may contribute to the efficiency of the cellulase mixture of Δ wc-1, Δ wc-2 or Δ vvd. Mutants were checked for known growth defects http://www.broadinstitute.org/annotation/genome/neurospora/MultiHome.html. Gene loci given in bold cause significantly altered specific cellulase activity or biomass accumulation on cellulose. Where available, data showed no indication of growth defects in the mutant strains used in this study