Figure 2

Results of qPCR (A) and microarray (B): expression changes of genes implicated in steroid metabolism (11-beta-hydroxysteroid dehydrogenase, HSD11B), formation of extracellular matrix (lumican, LUM; protein-lysine 6-oxidase, LYOX, SPARC precursor, SPRL1) and bone (mimecan or osteoglycin, OGN), contraction (enolase 3, ENOA, myosin regulatory light chain 2, MLC2). Cortisol injected fish (C0), cortisol injected and lice challenged fish (CL) and sham injected and lice challenged fish (SL) were compared to the sham injected group (S0). Data for qPCR are -ΔΔCt ± SE of 8 fish per group, 4 of which were used for the microarray analysis. Asterisks denote significant difference from S0 (P < 0.05). Data for microarrays are mean log2-ER ± SE of 4 fish hybridized to a common reference sample; sham injected control (S0) was subtracted from each group.