Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes

Table 3 qPCR validation of results and microarray data obtained from 29-50 hr larvae

		microarray		qPCR		microarray				qPCR
		Manf^Δ96 vs w		Manf^Δ96 vs w		Manf^Δ96vs 69B> Manf¹³³		69B> Manf¹³³ vs w		69B> Manf¹³³ vs w
#	Gene Name	log FC	P.Value	log2	T-test	log FC	P.Value	log FC	P,Value	log2	T-test	gene ID
1	DmManf			-8.0	0.0001	-2.0	0.0002			3.2	6.0E-07	CG7013
2	CG14879	-3.2	5.5E-06	-11.5	0.0002	-2.7	4.4E-05			-3.9	1.5E-06	CG14879
3	cycle	-0.5	0.0002	-7.2	0.0001	-1.0	4.7E-09	0.5	3.2E-05	-5.0	9.0E-08	CG8727
4	nol	-1.1	0.0003	-4.3	1.7E-05	-1.9	3.9E-07	1.1	6.3E-06	0.8	0.0005	CG32077
5	Pak3	-2.8	3.1E-07	-5.8	5.2E-05	-2.7	3.8E-07					CG14895
6	pipe	-0.9	0.0003	-1.2	3.7E-06	-1.0	1.8E-05	0.9	4.1E-05			CG9614
7	Rala	-1.4	2.4E-08	-2.0	0.0001	1.4	5.7E-09	0.9	4.2E-06			CG2849
8	Rep	-2.0	0.0001	-3.3	4.6E-07							CG8432
9	sip3	-0.5	0.0003	-9.6	6.4E-06							CG1937
10	Sk2	-0.6	0.0007	-8.7	1.2E-05	-1.6	0.0003	0.6	0.0003	-12.2	4.5E-08	CG32484
11	Ubc-E2H	0.7	0.0001	1.2	9.9E-05							CG2257
12	Pi3K	-1.0	5.5E-05	-9.9	0.0003			-1.0	8.0E-05			TC208938
13	Uch-L3	-0.6	0.0003	-0.4	0.0001	-0.4	0.0004					CG3431

Only statistically significant (P < 0.01) results are presented. Results of qPCR results were made comparable to microarray fold change (FC) by calculating the log2 of the relative fold changes. T-test means calculated P-value associated with Student's T-test.

ISSN: 1471-2164