Can it really achieve globin switching in vitro within one-phase system?
Wentian Wang, pumc
10 March 2013
see below
Competing interests
First, I can not blieve erythropoiesis under 34-day induction with high EPO and other cell factors.
In my experience, erythropoiesis will end in 2-3 weeks.it is too long to sustain differenetiation.
Second, in one-phase system, it can not realize globin switching spontaneously, at least I supposed so.
There is a incompatible result from another reference this year, in which the globin switching can only happen under feeder cell co-culture system.
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036846
So, which one is the right veritas?
Can it really achieve globin switching in vitro within one-phase system?
10 March 2013
see below
Competing interests
First, I can not blieve erythropoiesis under 34-day induction with high EPO and other cell factors. In my experience, erythropoiesis will end in 2-3 weeks.it is too long to sustain differenetiation. Second, in one-phase system, it can not realize globin switching spontaneously, at least I supposed so. There is a incompatible result from another reference this year, in which the globin switching can only happen under feeder cell co-culture system. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036846 So, which one is the right veritas?