Figure 5

Effect of miRNA inhibition on permeability of RAEC monolayer. Cells were transfected with specific inhibitors to miR-466d-5p and miR-466f-3p, or a scrambled negative control, and subjected to stretch or served as unstretched controls. Permeability is expressed as fluorescence of BODIPY-ouabain tracer above baseline. Results are reported as mean ± SE and are normalized to the reference bar (set at 1), which represents the fluorescence of unstretched, untransfected cells. There is a large increase in permeability with cyclic stretch, which is partly ameliorated by specific miRNA inhibition. N = 4/group. P values are results of ANOVA and post-hoc Tukey.