Nucleotide misincorporation patterns observed after trimming the first or the first two bases of sequencing reads. Nucleotide misincorporation patterns observed when using the reads recovered after trimming the first base (left) or the two first bases (right) of the sequencing reads, provided that in absence of trimming one or two successive nucleotide misincorporations would have been observed as a result of cytosine post-mortem deamination (see Methods). Red: C → T. Blue: G → A. Pink: Insertions. Green: Deletions. Orange: Clipped bases. Grey: Other misincorporations.