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Table 1 Number of reads obtained per experimental condition and their respective Newbler assembly results

From: Fast skeletal muscle transcriptome of the Gilthead sea bream (Sparus aurata) determined by next generation sequencing

Assembler Parameters Adult Fasted Juvenile 21°C Juvenile 30°C Juvenile 11°C Total assembly
Newbler v2.5 Reads 351895 486993 439734 459853 433264 2711149
  Reads assembled 166460 239792 189314 258331 200313 1157833
  Singletons 19809 24231 19140 21125 24896 96351
  Isotigs 6502 9152 8407 9922 9524 50515
  Isotigs over 100 bp 6254 8595 8242 9554 9267 43461
  Isotig mean lenght (bp) 875 589 936 551 550 454
  N50 (bp) 1092 732 1269 672 642 679
  Isotigs Annotated 2634 2791 3031 3211 3029 10465
  1. Singletons: reads not contained in the final assembly.
  2. Isotig: contigs consistently connected by a set of reads.
  3. N50: The value was computed by sorting all contigs from largest to smallest and by determining the minimum set of contigs whose sizes total 50% of the entire transcriptome.