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Table 5 Expression analysis of libraries showing isotigs where reads from each experimental condition significantly contributed to the assembly

From: Fast skeletal muscle transcriptome of the Gilthead sea bream (Sparus aurata) determined by next generation sequencing

Condition Isotig number Gene description Orthologue accession number e-value p-value FDR p-value
Fasted 21°C Isotig06049 Slow myosin heavy chain 2 CBN81811.1 0.0 0 0
  Isotig05152 Similar to ankyrin 2 CAM15089.1 2e-24 0 0
  Isotig01065 Calcium binding and coiled coil domain AAI17592.1 1e-57 0 0
  Isotig30481 myotubularin-related protein 5 NP_001038623 3e-11 0 0
  Contig01939 GTPase, IMAP family member 7 ACO08772.1 2e-53 0 0
  Isotig31931 Myosin, heavy polypeptide 6 CAX12653.1 2e-06 0 0
  Isotig06490 Jeltraxin ACN11240.1 6e-14 1,00e-05 0.004
  Isotig30481 Adenylate kinase 1-2 ACM41863.1 5e-33 0 0
  Isotig07982 Aurora kinase A-interactinng protein ACQ58398 4e-92 0 0
  Isotig06835 Slow Troponin T2 AAV80376.1 1e-68 0 0
Fed 21°C Contig02082 Parvalbumin ACM41857.1 0.72 0 0
  Isotig05339 VHSV-induced protein AEG78384 1e-15 1,00E-05 0.004
  Isotig17563 Putative nuclease HARBI1 XP_003200346.1 1e-16 0 0
  Isotig18811 Notch 2 BAA20535.1 9e-61 0 0
  Isotig22470 Phosphatidylinositol N-acetylglucosaminyltransferase NP_955461.1 7e-15 0 0
  Isotig20332 Glyceraldehyde 3-dehydrogenase XP_9741181.1 5e-14 0 0
  Contig01939 GTPase, IMAP family member 7 ACO08772.1 2e-53 0 0
  Isotig07008 Ribosomal protein L28 ACQ58416.1 5e-59 2,00E-05 0.009
  Isotig38944 AMP deaminase-1-like XP_003212994.1 3e-04 0 0
  Isotig28974 Myosin light chain 2 AAX34414.1 2e-10 8,00E-05 0.03
33°C Isotig05428 Xin actin-binding repeat containing protein 1 NP_001012377.1 0.0 0 0
  Isotig02673 Clusterin-1 NP_001117890.1 3e-139 0 0
  Isotig02981 Myosin-6-like isoform 1 XP_001923213.1 0.0 0 0
  Isotig08301 Heat shock protein 30 NP_001134440.1 6e-62 0 0
  Isotig05469 Activator of 90kda heat shock protein ATPase homolog 1 NP_997767.1 2e-145 5,00E-05 0.02
  Isotig01745 Selenoprotein L NP_001180385.1 3e-63 8.00E-05 0.03
  Isotig07184 eEF1A2 binding protein NP_001133224.1 1e-104 0 0
  Isotig03041 Heat shock protein 4a NP_999881.1 0.0 5,00E-05 0.02
  Isotig18085 Srfs18 CAG06353.1 1e-45 1,00E-05 0.004
  Isotig02073 Heat shock protein 90 AAQ95586.1 0.0 0 0
11°C Isotig05303 Zinc binding protein 33A XP_694642.3 5e-61 0 0
  Isotig01520 Interferon stimulated gene 15 BAJ16365.1 4e-46 0 0
  Isotig03692 Receptor transporting protein 3 ACQ57966.1 6e-66 0 0
  Isotig03214 Nicotinic acetylcholine receptor alpha 1b CAG09972.1 0.0 0 0
  Isotig05152 Similar to ankyrin 2 CAM15089.1 2e-24 0 0
  Isotig06087 G-rich sequence factor 1 NP_001135339.1 1e-107 0 0
  Isotig07416 Presenilin associated ABG81447.1 6e-45 1,00E-05 0.004
  Isotig05735 Ubtf protein AAI15119.1 5e-169 0 0
  Isotig19061 rRNA promoter binding protein NP_671477 7e-10 0 0
  Isotig06639 C6orf64 ACO14504.1 2e-26 0 0
  1. Gene description, orthologue accession number and e-values were obtained by blastx against the NCBI nr database. Both p-values and FDR p-value were calculated by chi-square and FDR statistic using R statistical package [70].