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Table 5 Expression analysis of libraries showing isotigs where reads from each experimental condition significantly contributed to the assembly

From: Fast skeletal muscle transcriptome of the Gilthead sea bream (Sparus aurata) determined by next generation sequencing

Condition

Isotig number

Gene description

Orthologue accession number

e-value

p-value

FDR p-value

Fasted 21°C

Isotig06049

Slow myosin heavy chain 2

CBN81811.1

0.0

0

0

 

Isotig05152

Similar to ankyrin 2

CAM15089.1

2e-24

0

0

 

Isotig01065

Calcium binding and coiled coil domain

AAI17592.1

1e-57

0

0

 

Isotig30481

myotubularin-related protein 5

NP_001038623

3e-11

0

0

 

Contig01939

GTPase, IMAP family member 7

ACO08772.1

2e-53

0

0

 

Isotig31931

Myosin, heavy polypeptide 6

CAX12653.1

2e-06

0

0

 

Isotig06490

Jeltraxin

ACN11240.1

6e-14

1,00e-05

0.004

 

Isotig30481

Adenylate kinase 1-2

ACM41863.1

5e-33

0

0

 

Isotig07982

Aurora kinase A-interactinng protein

ACQ58398

4e-92

0

0

 

Isotig06835

Slow Troponin T2

AAV80376.1

1e-68

0

0

Fed 21°C

Contig02082

Parvalbumin

ACM41857.1

0.72

0

0

 

Isotig05339

VHSV-induced protein

AEG78384

1e-15

1,00E-05

0.004

 

Isotig17563

Putative nuclease HARBI1

XP_003200346.1

1e-16

0

0

 

Isotig18811

Notch 2

BAA20535.1

9e-61

0

0

 

Isotig22470

Phosphatidylinositol N-acetylglucosaminyltransferase

NP_955461.1

7e-15

0

0

 

Isotig20332

Glyceraldehyde 3-dehydrogenase

XP_9741181.1

5e-14

0

0

 

Contig01939

GTPase, IMAP family member 7

ACO08772.1

2e-53

0

0

 

Isotig07008

Ribosomal protein L28

ACQ58416.1

5e-59

2,00E-05

0.009

 

Isotig38944

AMP deaminase-1-like

XP_003212994.1

3e-04

0

0

 

Isotig28974

Myosin light chain 2

AAX34414.1

2e-10

8,00E-05

0.03

33°C

Isotig05428

Xin actin-binding repeat containing protein 1

NP_001012377.1

0.0

0

0

 

Isotig02673

Clusterin-1

NP_001117890.1

3e-139

0

0

 

Isotig02981

Myosin-6-like isoform 1

XP_001923213.1

0.0

0

0

 

Isotig08301

Heat shock protein 30

NP_001134440.1

6e-62

0

0

 

Isotig05469

Activator of 90kda heat shock protein ATPase homolog 1

NP_997767.1

2e-145

5,00E-05

0.02

 

Isotig01745

Selenoprotein L

NP_001180385.1

3e-63

8.00E-05

0.03

 

Isotig07184

eEF1A2 binding protein

NP_001133224.1

1e-104

0

0

 

Isotig03041

Heat shock protein 4a

NP_999881.1

0.0

5,00E-05

0.02

 

Isotig18085

Srfs18

CAG06353.1

1e-45

1,00E-05

0.004

 

Isotig02073

Heat shock protein 90

AAQ95586.1

0.0

0

0

11°C

Isotig05303

Zinc binding protein 33A

XP_694642.3

5e-61

0

0

 

Isotig01520

Interferon stimulated gene 15

BAJ16365.1

4e-46

0

0

 

Isotig03692

Receptor transporting protein 3

ACQ57966.1

6e-66

0

0

 

Isotig03214

Nicotinic acetylcholine receptor alpha 1b

CAG09972.1

0.0

0

0

 

Isotig05152

Similar to ankyrin 2

CAM15089.1

2e-24

0

0

 

Isotig06087

G-rich sequence factor 1

NP_001135339.1

1e-107

0

0

 

Isotig07416

Presenilin associated

ABG81447.1

6e-45

1,00E-05

0.004

 

Isotig05735

Ubtf protein

AAI15119.1

5e-169

0

0

 

Isotig19061

rRNA promoter binding protein

NP_671477

7e-10

0

0

 

Isotig06639

C6orf64

ACO14504.1

2e-26

0

0

  1. Gene description, orthologue accession number and e-values were obtained by blastx against the NCBI nr database. Both p-values and FDR p-value were calculated by chi-square and FDR statistic using R statistical package [70].