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Table 1 Numbers of essential genes under laboratory conditions in relevant E. coli , S. Typhimurium and S. Typhi isolates

From: High-throughput comparison of gene fitness among related bacteria

Species/serovar Strain Essential genes/non- essential Method (type of mutagenesis, medium) Reference
E. coli K-12 MG1655 302/4477 Published literature and MD (medium-scale) and LD (large-scale) deletion mutants (targeted mutagenesis, antibiotic medium 3) Profiling of E. coli chromosome (PEC) database ([9, 10]
E. coli K-12 BW25113 303/3985 Single-gene deletion mutants (targeted mutagenesis, LB) Keio collection[7]
E. coli K-12 BW25113 299/3864 Single-gene deletion mutants (targeted mutagenesis, LB) Update on the Keio collection[8]
E. coli K-12 W3110 299/4109 Published literature PEC database ([9, 10]
S. Typhimurium ATCC 14028 NA/1,023 Single-gene deletion mutants (targeted mutagenesis, LB) [4]
S. Typhimurium ATCC 14028 257/NA Insertion-duplication mutagenesis (IDM) sequencing (random mutagenesis, LB) [5]
S. Typhimurium LT2 144 (LB and/or M9/glc)/NA Metabolic reconstruction (in silico approach, M9/glc and LB) [6]
S. Typhi Ty2 (STY2) 356/4162 Random transposon mutagenesis and two types of growtha [3]
  1. a Plating on an “aro mix” agar containing L-phe, L-trp, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid (condition 1), six passages of growth in Luria broth (condition 2).