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Table 4 Results of SNP validation using SNaPshotâ„¢ and GoldenGateâ„¢ chemistries including a comparison of the genotype calls

From: Genome-wide SNP identification in multiple morphotypes of allohexaploid tall fescue (Festuca arundinacea Schreb)

SNP class and sub-class (GoldenGate clustering)

Number of SNPs

% genotypes with concordant allele calls

SNaPshot

GoldenGate

Compared between genotyping systems1

Polymorphic

70

62

38

80.45

  3 clusters  (1 sub-genome amplified)

N/A

17

12

87.01

  3 clusters  (3 sub-genomes amplified)

N/A

11

5

86.00

  2 clusters

N/A

20

13

80.15

  5 clusters

N/A

2

1

48.65

  Dispersed

N/A

12

7

71.71

Diagnostic

4

3

1

97.67

Monomorphic

15

16

7

39.11

Failed

7

14

5

N/A

Total

96

96

51

76.43

  1. 1 SNPs are classed according to the GoldenGateâ„¢ results.