Widespread gene expression uncoupling is a general and recurring phenomenon in all transcriptome-translatome profiling datasets. (A) Barplot displaying the degree of uncoupling between transcriptome and translatome DEGs for each dataset. Collected datasets are labelled by short names as explained in Table 1. Bar lengths show the relative proportion of DEGs in the four classes defined in Table 1. The corresponding percentages of uncoupled DEGs are shown on the right. (B) Uncoupling estimate is independent from the significance threshold and the algorithm used for calling DEGs. Percentage of DEGs detected by the comparison (homodirectional change in green, antidirectional change in red) between both transcriptome and translatome profiles, DEGs detected by the transcriptome comparison only (in cyan) and DEGs detected by the translatome comparison only (in yellow) were computed over all the datasets described in Table 1. Three algorithms are shown: RankProd, t-test and SAM. Inside each barplot the significance thresholds ranges from 0.01 to 0.5. In the barplot generated with RankProd the red vertical dashed line indicates the 0.2 significance threshold used to detect DEGs throughout the analysis. For t-test and SAM a Benjamini-Hochberg multiple test correction was applied to the resulting p-values.