Marker-trait association in the Hls locus. The Hls locus on the cowpea consensus genetic map linkage group 4 is depicted vertically along with cowpea genotypes which differ in hastate or sub-globose leaf shape. Red colored blocks indicate the “AA” allele, blue colored blocks indicate the “BB” allele and grey colored blocks indicate that the locus has no detected SNP. Leaf shapes for cowpea accessions are labeled below: “S” indicates a sub-globose leaf shape and “H” indicates the hastate leaf shape. A marker-trait association was found for SNP marker 1_0349 (35.90 cM position) which is boxed in green. SNP marker 1_0349 co-segregated with the hastate and sub-globose leaf genotypes and the corresponding leaf phenotype. The allele for the hastate leaf genotype at this locus is the thymine nucleotide, color coded blue. The allele for the sub-globose genotype is the cytosine nucleotide, color coded red. The thymine/cytosine SNP for 1_0349 is at position 2122 in the cowpea P12 assembly unigene 8605 and can be viewed in HarvEST:Cowpea (http://harvest.ucr.edu).