Figure 3

Synteny of the Hls locus with Medicago truncatula and Glycine max . Synteny was examined for the Hls locus between cowpea and M. truncatula and cowpea and G. max using EST-derived SNP markers previously BLASTed and aligned to the sequenced genomes. The Hls locus which spans 25.57 cM to 35.96 cM on linkage group 4 of the cowpea consensus genetic map was syntenic with Medicago chromosome 7. The syntenic locus spanned from Medicago locus Medtr7g084010 to Medtr7g134530. A candidate gene was identified in the highly significant syntenic region of Hls, Medtr7g133020, which was annotated as an ortholog of the Arabidopsis EZA1/SWINGER (SWN) gene. Two syntenic loci were identified for the Hls locus in soybean chromosomes 3 and 19. The syntenic region in soybean chromosome 3 spanned from the soybean locus Glyma03g34240 to Glyma03g38550. An orthologous candidate gene was observed in the most significant region of the syntenic Hls locus, Glyma03g38320, which was annotated as an ortholog of the Arabidopsis EZA1/SWINGER (SWN) gene. The syntenic Hls locus on soybean chromosome 19 spanned from Glyma19g36180 to Glyma19g41150 where another soybean ortholog of the EZA1/SWINGER (SWN) gene, Glyma19g40430, was observed. The syntenic map was drawn using HarvEST:Cowpea database (http://harvest.ucr.edu) using a cut off e-score value of −10 and a minimum number of 10 lines drawn per linkage group. Colored lines indicate cowpea genes orthologous to genes on M. truncatula and G. max chromosomes.