Larval midgut modifications associated with Bti resistance in the yellow fever mosquito using proteomic and transcriptomic approaches

Table 4 Altered expression of known Bti Cry-binding proteins detected by transcriptomic and proteomic approaches

Gene	Accession number	Microarrays	RT-qPCR	2D-DIGE	Binding protein	Ref
Alkaline phosphatase (ALP1)	AAEL000931	ND	+11.40	NI	Cry11Aa	[33]
Alkaline phosphatase (ALP2)	AAEL003298	−1.57	−1.56	−1.92; −1.16	Cry4Ba	[29]
Alkaline phosphatase (ALP3)	AAEL003313	+1.15	+1.52	−1.58	Cry4Ba	[29]
Alkaline phosphatase (ALP4)	AAEL009077	ND	+1.15	NI	Cry11Aa	[36]
Alkaline phosphatase (ALP5)	AAEL015070	+3.95	+7.69	NI	Cry4Ba	[29]
Cadherin (Cad1)	AAEL007478	ND	−1.11	UD	Cry11Aa	[34]
Cadherin (Cad2)	AAEL007488	−1.47	−1.46	UD	Cry11Aa	[34]
N-Aminopeptidase (APN1)	AAEL012774	+1.44	+1.26	+1.80; +2.34	Cry11Aa	[35]
N-Aminopeptidase (APN2)	AAEL012776	−1.34	−1.62	+1.81; −2.74; −3.81	Cry4Ba	[29]
N-Aminopeptidase (APN3)	AAEL012778	−1.04	+1.20	NI	Cry11Aa	[35]

Given values indicate the level of expression in the LiTOX strain compared to the susceptible strain detected in microarrays, RT-qPCR and DIGE experiments. ND, Non detected in at least 5 of the 6 microarray hydribizations; NI, Non identified as differentially expressed between the two strains and therefore non-picked for MS/MS identification; UD, Undetectable in 2D-DIGE due to their high molecular weight and their low amount in BBMV.

ISSN: 1471-2164