Figure 6

The S . Typhimurium pykF gene is expressed from alternative ppGpp-dependent and independent promoters. Enriched (+) and non-enriched (-) cDNAs of S. Typhimurium wild-type (black) or ΔrelAΔspoT (red) strains mapped onto the pykF gene (encoding pyruvate kinase). The Y axis in each lane represents 0-80 mapped reads per genome position. The genome co-ordinates are shown across the top. Enriched reads show the presence of alternative TSSs in the wild-type strain (black arrow) and the ΔrelAΔspoT strain (red arrow). A possible third TSS showing equal read numbers in both wild-type and ΔrelAΔspoT strains is indicated by a shorter black arrow.