Figure 4

Relative expression profile of transcripts from the 14-gene survival signature following oyster challenge. The two types of expression profiles obtained are illustrated taking 4 genes among the signature, as examples. Hemocyte gene expression was analyzed by qPCR in unchallenged oysters (white bars) and in oysters 24 h after Vibrio splendidus LGP32 (black bars) or sterile sea water (grey bars) injection. Results are presented as mean values from 10 individuals per conditions. Relative expression levels were calculated according to the 2^-ΔΔCq method normalized with the ribosomal L40 protein (Cg-rpl40). Asterisks (*) indicate significant differences of gene expression between conditions (P < 0.05).