Figure 3

dRif1 localizes to the nucleus in unperturbed cells. A) Western blot of total protein extract from S2 cells was probed with antibody against dRif1. A 160 kDa lights up prominently. B) S2 cells were immunostained with antibodies to dRif1 (red) and costained with antibodies to lamin (green) to mark the nucleus. C) S2 cells were immunostained with antibodies to H3K9 trimethyl (green) and dRif1 (red) and TO-PRO3 to mark the nucleus (scale bar, 5 μm).