Effect of osmotic stressors, antifungal agents and oxidative stress on the growth of Arl1 overexpressing and wild type cells. A) Arl1 overexpression confers resistance to COS-5.44 at a concentration that inhibits growth of the vector control cells. The Arl1 overexpressing strain is as sensitive as the wild type cells to the tested antifungal compounds: B) Amphotericin B; C D) Fluconazole; E F) Terbinafine. Overexpression of ARL1 confers tolerance to H2O2 (G) compared with the wild type. All assays were done in YPD except COS-5.44 that was done in 0.5X YPD. Optical density readings were taken every 15 min over 20 hrs using a Tecan Genios reader. Tecan ODs were converted to conventional 1 mm path length cuvette ODs using a calibration function provided by Ericson et al. 2010, . Three colonies of each strain were grown in triplicate and compared with the wild type grown under the same conditions. YPD - Yeast Peptone Dextrose Broth.