Figure 7From: Identification of yeast genes that confer resistance to chitosan oligosaccharide (COS) using chemogenomicsEffect of osmotic stressors, antifungal agents and oxidative stress on the growth of Arl1 overexpressing and wild type cells. A) Arl1 overexpression confers resistance to COS-5.44 at a concentration that inhibits growth of the vector control cells. The Arl1 overexpressing strain is as sensitive as the wild type cells to the tested antifungal compounds: B) Amphotericin B; C D) Fluconazole; E F) Terbinafine. Overexpression of ARL1 confers tolerance to H2O2 (G) compared with the wild type. All assays were done in YPD except COS-5.44 that was done in 0.5X YPD. Optical density readings were taken every 15 min over 20 hrs using a Tecan Genios reader. Tecan ODs were converted to conventional 1 mm path length cuvette ODs using a calibration function provided by Ericson et al. 2010, [24]. Three colonies of each strain were grown in triplicate and compared with the wild type grown under the same conditions. YPD - Yeast Peptone Dextrose Broth.Back to article page