Table 1 Top 39 characterized sensitive deletion strains found in the HIP-HOP assays above 3.5 log 2 ratio
probeid | COS-5.44 (91.1 μg/ml) (log2ratio) | Gene | SGD_description | Fitness score (avg)2 | Deletion pool source3 |
|---|---|---|---|---|---|
YBR 164C | 4.686 | ARL1 | Soluble GTPase of the RAS superfamily, with a role in regulation of membrane traffic mainly regulates pottassium influx; similar to ADP-ribosylation factor. | 0.756 | HOM |
YBR257W | 3.534 | POP4 | subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5′ends; binds to the RPR1 RNA subunit in RNase P. | 0.9963 | HET |
YDL060W | 3.769 | TSR1 | Protein required for processing of 20S pre-rRNA in the cytoplasm associates with pre-40S ribosomal particles. | 0.8887 | HET |
YDL213C | 5.101 | NOP6 | rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM). | 0.8854 | HOM |
YDR300C | 4.371 | PRO1 | Gamma-glutamyl kinase, catalyzes the first step in proline biosynthesis. | 0.8521 | HOM |
YDR320C | 3.571 | SWA2 | Auxilin-like protein involved in vesicular transfort; clathrin-binding protein required for uncoating of clathrin-coated vesicles | 0.9621 | HOM |
YDR324C | 3.636 | UTP4 | Subunit of U3 involved in production of 18S rRNA, assembly of small ribosomal subunit and transcription of 35S rRNA transcript, member of t-Utp subcomplex. | 0.761 | HET |
YDR389W | 4.281 | SAC7 | GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate. | 0.8768 | HOM |
YDR455C | 3.577 | YDR455C | Partially overlaps the verified gene YDR456W. | 0.9312 | HOM |
YGL045W | 4.229 | RIM8 | Protein involved in proteolytic activation of Rim 101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; member of the arrestin-related trafficking adaptor family. | 0.896 | HOM |
YGR056W | 3.72 | RSG1 | Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes. | 0.8656 | HOM |
YGR122W | 3.719 | YGR122W | Probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim 101p and has decreased resistance to rapamycin. | 0.9913 | HOM |
YGR246C | 3.887 | BRF1 | TFFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB. | 0.9782 | HET |
YHL009C | 3.625 | YAP3 | Basic leucine zipper (bZIP) transcription factor. | 0.8854 | Â |
YIL019C | 3.59 | FAF1 | Protein required to pre-rRNA processing and 40S ribosomal subunit assembly. | 0.9603 | HET |
YIL022W | 3.931 | TIM44 | Essential component of the Translocase of the inner Metochondrial membrane; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex). | 0.8167 | HET |
YIL048W | 3.83 | NEO1 | Putative aminophospolipid translocase (flippase) involved in endocytosis and vacuolar biogenesis. | 1.0734 | HET |
YIL075C | 3.653 | RPN2 | Subunit of the 26Sproteasome, substrate of the N-acetyltransferase Nat1p. | 0.9832 | HET |
YIL154C | 4.315 | IMP2 | Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants. | 0.2516 | HOM |
YJL002C | 3.515 | OST1 | Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins. | 1.0555 | HET |
YGL188C | 3.656 | BUD19 | ORF overlaps the verified gene RPL39 by 88%f; diploid mutants displays a weak budding pattern phenotype in a systematic assay. | 1.0087 | HOM |
YJR102C | 3.755 | VPS25 | Component of the ESCRT-II complex, which is involved in ubiquitin -dependent sorting of proteins into the endosome. | 1.0011 | HOM |
YKL114C | 4.271 | APN1 | Apurinic/apyrimidinic endonuclease, 3′-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3′-5′ exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine. | 0.8878 | HOM |
YKR024C | 3.506 | DPB7 | Putative ATP-dependent RNA helicase of the dead -box family involved in ribosomal biogenesis. | 0.9795 | HOM |
YKR025W | 4.622 | SNF7 | One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway. | 0.8599 | HOM |
YLR100W | 3.929 | ERG27 | 3-keto sterol reductase, catalyzes of the last three steps required to removed two C-4 methyl groups from an intermediate in engosterol biosynthesis; mutants are sterol auxotrophs. | 0.8809 | HET |
YLR147C | 3.525 | SMD3 | Core Sm protein Sm D3; part of heteroheptameric complex that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3. | 1.0582 | HET |
YLR223C | 3.633 | IFM1 | Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing. | 0.9691 | HET |
YLR378C | 3.539 | SEC61 | Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); with Sec63 complex forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER. | 0.8717 | HET |
YNL025C | 3.581 | SSN8 | Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain, in glucose repression and telomere maintenance. | 0.823 | HOM |
YNL287W | 3.66 | SEC21 | Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo. | 0.8959 | HET |
YNR046W | 4.047 | TRM112 | Subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the temary complex eRF1-eRF3-GTP. | 0.9906 | HET |
YOR014W | 3.844 | RTS1 | B-type regulatory subunit of protein phospatase 2A (PP2A); homolog of the mammalian B’subunit of PP2A. | 0.9832 | HOM |
YOR030W | 3.669 | DFG16 | Probable multiple transmembrane protein, involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; required for accumulation of processed Rim101p. | 1.0307 | HOM |
YOR080W | 4 | DIA2 | Origin-binding F-box protein that forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication, involved in invasive and pseudohyphal growth. | 1.1312 | HOM |
YOR117W | 3.506 | RPT5 | One of the six ATPases of the 19S regulatory particle (26S proteasome) involved in the degradation of ubiquintinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1. | 0.8955 | HET |
YOR244W | 3.5 | ESA1 | Catalytic subunit of the histone acetyltransferase complex (NuA4) that acetylates four conserved internal lysines of histone H4N-terminal tail; required for cell cycle progression and transcriptional silencing at the rDNA locus. | 0.8716 | HET |
YPL002C | 3.679 | SNF8 | Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression. | 0.8925 | HOM |
YPR139C | 3.643 | VPS66 | Cytoplasmic protcin of unknown function involved in vacuolar protcin sorting. | 0.8768 | HOM |