Celiac disease T-cell epitopes from gamma-gliadins: immunoreactivity depends on the genome of origin, transcript frequency, and flanking protein variation

Table 3 The number of CD-epitope cores in γ-gliadin transcripts of T. aestivum

epitope	Gli-A1	Gli-A1	Gli-B1	Gli-B1	Gli-B1	Gli-D1	Gli-D1	∑
	Group 6	Group 7	Group 3	Group 5	Group 8	Group 9	Group 10
DQ2-γ-VIIb^3*	701	144	72	230	57	1257	50	2511
DQ2-γ-VI³	224	0	0	0	38	678	50	990
DQ2-glia-γ2a⁶	140	30	36	59	38	684	0	987
DQ2-γ-II(Glia γ30)^3,4	136	0	41	125	0	293	61	656
DQ2-γ-I (Glia-γ1)²	120	38	41	115	19	293	61	567
DQ2-γ-VIIa³	0	0	0	115	19	0	0	134
DQ2-γ-IV⁵	0	0	0	0	0	0	61	61
DQ2-γ-III^5*	0	0	0	0	0	0	50	50
DQ2-glia-γ2b⁶	0	0	0	0	0	0	50	50
N_epitopes	1321	212	190	644	171	3205	383	6006
N_transcripts	*140*	38	41	*125*	19	*293*	61	717
N_epitopes/N_transcripts	9.4	5.6	4.6	5.2	9.0	10.9	6.3	8.4
N_epitopes/N_transcripts	8.6 (1533/178)		5.4 (1005/185)			10.1 (3588/354)

The number of T-cell stimulating sequences involved in CD (9 mer epitope cores) found in a set of 717 γ-gliadin transcripts from T. aestivum (genome ABD) attributed to respectively Gli-A1 (topology group 6 and 7), Gli-B1 (topology group 3, 5, and 8) and Gli-D1 (topology group 9 and10). The sequence topology was obtained by Neighbor joining analysis (see Figure 2). The frequency of T-cell epitopes in γ- gliadin transcripts (N_epitopes/N_transcripts) is calculated.

ISSN: 1471-2164