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Table 1 Sequence Statistics and RNA qualities

From: Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA

Samples Non- globin reduced Globin-reduced
  1 2 3 4 5 6 1 2 3 4 5 6
RIN 8.1 7.7 7.1 6.9 7.3 8.8 7.6 7.2 6.7 6.9 7 8.2
Tag numbers 29.2.106 13.2.106 32.0.106 11.6.106 13.6.106 10.1.106 16.5.106 28.7.106 15.9.106 15.3.106 15.9.106 14.9.106
Non- aligned 1.27% 5.63% 1.43% 13.80% 5.64% 1.92% 3.64% 2.95% 3.37% 3.29% 3.45% 3.38%
Aligned 89.9% 84.4% 89.9% 80.1% 89.1% 91% 78.3% 78.9% 78.4% 78.9% 77.6% 77.5%
Globin tags 60.1% 52.1% 57.2% 69.6% 76% 71% 0.3% 0.4% 0.4% 0.2% 0.4% 0.1%
  1. Quality of RNA depicted by RNA integrity numbers (RIN) for the 6 non-globin reduced and globin reduced samples. The number of tags, percentages of aligned and non-aligned sequences, and relative number of globin tags are shown as percentage of the total number of aligned tags.