Table 2 Polymorphic SSRs primer pairs derived from N. nervosa unigenes
ID name | Locus | Repeat motif | ORF | Forward and Reverse Primers | Amplicon length observed | BLASTX, seq description | Seq Lenght (bp) | Sim mean (%) | GO terms related to response to stress |
|---|---|---|---|---|---|---|---|---|---|
isotig00192 | INTANOT1 | (tct)5 | Y | F: CCAGATGGGTTTTTGCTTGT | 148 | heat shock protein 81-1 | 2309 | 97.2 | response to stimulus |
R: GACGATGAAGACGATGAGC | |||||||||
isotig00230 | INTANOT2 | (tcg)5 | N | F: TTTCCAAACGGTTCCAGAAG | 120 | af367280_1at3g56860 t8m16_190 | 1229 | 76.6 | response to stress |
R:AACGGAGAAGGATGTTTCCA | |||||||||
isotig00551 | INTANOT3 | (tcattt)3 | Y | F: CCGATGTGATCGATAGGCTT | 204 | ac005850_9highly simlilar to mlo proteins | 1759 | 77.5 | defense response to fungus |
R: CATGTCCCCAGTTCACCTCT | |||||||||
isotig00597 | INTANOT4 | (ta)6 | N | F:AAAACACCACCAAACCCAAA | 197 | dnaj heat shock n-terminal domain-containing protein | 1516 | 78.3 | response to stimulus |
R: CTTTGCCACGGCAACTAAAT | |||||||||
isotig01207 | INTANOT5 | (tct)7 | N | F: CTCGAAGACGCTACCAGACC | 280 | af214107_1 -like protein | 748 | 79.3 | response to stimulus |
R: TCCTGGGTTTTGCATATTGG | |||||||||
isotig01232 | INTANOT6 | (atc)4 | Y | F: CGTTTCCCTTTAGCTGATGC | 173 | aldh6b2 3-chloroallyl aldehyde dehydrogenase methylmalonate-semialdehyde dehydrogenase oxidoreductase | 741 | 96.8 | response to stress |
R:GCTGAGTTAGCAATGGAGGC | |||||||||
GR7D2IN01BK031 | INTANOT7 | (ag)5 | N | F: GACGACATCGTTCCGAGTTT | 241 | f-box family protein | 536 | 75.4 | response to heat |
R: GTTAATCCCTCTCTCCTCAT | |||||||||
GR7D2IN01CGQUT | INTANOT8 | (ccgaaa)3 | Y | F: CTCCCTCAAACACCTCCAAA | 236 | mitogen-activated protein kinase kinase | 518 | 90.5 | response to osmotic stress |
R: ATTCAAGTGGGTCTTGCCTG | |||||||||
GR7D2IN01EMGE0 | INTANOT9 | (ct)8 | N | F: CCGGCTACCTGTTTGTTTTA | 155 | at1g78870 f9k20_8 | 507 | 100.0 | response to metal ion |
R: TTCCTTGATGATTCTTCGGG | |||||||||
GR7D2IN02FPPC7 | INTANOT10 | (ggt)6 | Y | F: AAAATTGCTGTTGAGGGTGG | 117 | af361609_1at1g27760 t22c5_5 | 529 | 87.9 | response to osmotic stress |
R: CCTGAATCACCAGACCGAC | |||||||||
GR7D2IN02GFAUT | INTANOT11 | (gaa)4 | Y | F: ATCCCCAATCTTTCCCAATC | 115 | salt overly sensitive 1 | 315 | 78.5 | response to reactive oxygen species; response to osmotic stress |
R: AATTCTGTCCGCTTTGGCTA | |||||||||
GR7D2IN02GR6NZ | INTANOT12 | (at)5 | Y | F: TCTTGTGGCAAGTGCTTGAG | 285 | win2_soltu ame: full = wound-induced protein win2 flags: precursor | 472 | 94.0 | defense response |
R: ACTATCCTCACCGTTGCCTG | |||||||||
GR7D2IN02HOKOI | INTANOT13 | (tc)5 | Y | F: ATATCCTGGAAATGCTTGCG | 124 | exec1_arath ame: full = protein executer chloroplastic flags: precursor | 469 | 71.7 | response to reactive oxygen species |
R: TAAACGATCTTCGGAATGGC | |||||||||
GR7D2IN02HWXOR | INTANOT14 | (tgg)8 | Y | F: AGGAGCTAAATGGGCGTAA | 260 | glycine-rich rna-binding protein | 452 | 86.5 | response to stress |
R: CACCACCACCACCAAAGAA |