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Table 5 Verification of the changes in gene expression by quantitative realtime RT-PCR

From: Microarray analysis on germfree mice elucidates the primary target of a traditional Japanese medicine juzentaihoto: acceleration of IFN-α response via affecting the ISGF3-IRF7 signaling cascade

Gene Name Gene Symbol Gene ID Effect of JTX (Fold Change)* RT-PCR
    SPF GF primer/probe ID
Interferon-reguratory factors      
   interferon regulatory factor 1 (IRF1) Irf1 16362 1.18 1.00 Mm00515191_m1
   interferon regulatory factor 3 (IRF3) Irf3 54131 1.00 1.18 Mm00516779_m1
   interferon regulatory factor 5 (IRF5) Irf5 27056 3.79 1.46 Mm00496477_m1
   interferon regulatory factor 6 (IRF6) Irf6 54139 1.09 1.35 Mm00516797_m1
   interferon regulatory factor 7 (IRF7) Irf7 54123 2.81 0.33 Mm00516788_m1
   interferon dependent positive acting transcription factor 3 gamma(IRF9) Isgf3g 16391 1.99 0.73 Mm00492679_m1
   interferon consensus sequence binding protein 1(IRF8) Irf8 15900 1.00 1.40 Mm00492567_m1
Interferon-inducible genes      
   interferon-induced protein with tetratricopeptide repeats 1 Ifit1 15957 4.67 0.30 Mm00515153_m1
   interferon-induced protein with tetratricopeptide repeats 2 Ifit2 15958 3.79 0.29 Mm00492606_m1
   glucocorticoid-attenuated response gene 49 (GARG-49/IRG2) Ifit3 15959 6.13 0.27 Mm01704846_s1
   guanylate nucleotide binding protein 2 (mGBP-2) Gbp2 14469 1.10 1.03 Mm00494575_m1
   ISG15 ubiquitin-like modifier G1p2 53606 6.00 0.23 Mm01705338_s1
   chemokine (C-X-C motif) ligand 10 (IP-10) Cxcl10 15945 2.46 0.45 Mm00445235_m1
   interferon inducible protein 1 (Ifi1) Irgm 15944 4.54 0.94 Mm00492596_m1
   lectin, galactose binding, soluble 9 Lgals9 16859 2.53 0.58 Mm00495295_m1
   interferon gamma induced GTPase Igtp 16145 2.37 0.42 Mm00497611_m1
   lymphocyte antigen 6 complex, locus A (Ly6E.1, etc.) Ly6a 110454 2.25 0.39 Mm00726565_s1
   2'-5' oligoadenylate synthetase 1A Oas1a 246730 3.00 NT Mm00836412_m1
   protein kinase, interferon inducible double stranded RNA dependent Prkr 19106 2.02 NT Mm00440966_m1
   SAM domain and HD domain, 1 (Mgl1) Samhd1 56045 1.25 1.62 Mm00490121_m1
Type I interferon receptors      
   interferon (alpha and beta) receptor 1 Ifnar1 15975 1.04 1.60 Mm00439544_m1
   interferon (alpha and beta) receptor 2 Ifnar2 15976 0.97 1.44 Mm00494916_m1
Type 1 interferons      
   MuIFN-alpha-2 interferon-alpha-2 gene Ifna2 15965 ND NT Mm00833961_s1
   Interferon alpha family, gene 4 Ifna4 15967 ND NT Mm00833969_s1
Stat family      
   Signal transducer and activator of transcription 1(Stat1) Stat1 20846 2.96 NT Mm00439518_m1
   Signal transducer and activator of transcription 2(Stat2) Stat2 20847 4.24 NT Mm00490880_m1
  1. * Fold changes statistically significant (P < 0.05) are indicted in bold letter. NT: not tested.