Table 5 Verification of the changes in gene expression by quantitative realtime RT-PCR
Gene Name | Gene Symbol | Gene ID | Effect of JTX (Fold Change)* | RT-PCR | |
|---|---|---|---|---|---|
SPF | GF | primer/probe ID | |||
Interferon-reguratory factors | |||||
interferon regulatory factor 1 (IRF1) | Irf1 | 16362 | 1.18 | 1.00 | Mm00515191_m1 |
interferon regulatory factor 3 (IRF3) | Irf3 | 54131 | 1.00 | 1.18 | Mm00516779_m1 |
interferon regulatory factor 5 (IRF5) | Irf5 | 27056 | 3.79 | 1.46 | Mm00496477_m1 |
interferon regulatory factor 6 (IRF6) | Irf6 | 54139 | 1.09 | 1.35 | Mm00516797_m1 |
interferon regulatory factor 7 (IRF7) | Irf7 | 54123 | 2.81 | 0.33 | Mm00516788_m1 |
interferon dependent positive acting transcription factor 3 gamma(IRF9) | Isgf3g | 16391 | 1.99 | 0.73 | Mm00492679_m1 |
interferon consensus sequence binding protein 1(IRF8) | Irf8 | 15900 | 1.00 | 1.40 | Mm00492567_m1 |
Interferon-inducible genes | |||||
interferon-induced protein with tetratricopeptide repeats 1 | Ifit1 | 15957 | 4.67 | 0.30 | Mm00515153_m1 |
interferon-induced protein with tetratricopeptide repeats 2 | Ifit2 | 15958 | 3.79 | 0.29 | Mm00492606_m1 |
glucocorticoid-attenuated response gene 49 (GARG-49/IRG2) | Ifit3 | 15959 | 6.13 | 0.27 | Mm01704846_s1 |
guanylate nucleotide binding protein 2 (mGBP-2) | Gbp2 | 14469 | 1.10 | 1.03 | Mm00494575_m1 |
ISG15 ubiquitin-like modifier | G1p2 | 53606 | 6.00 | 0.23 | Mm01705338_s1 |
chemokine (C-X-C motif) ligand 10 (IP-10) | Cxcl10 | 15945 | 2.46 | 0.45 | Mm00445235_m1 |
interferon inducible protein 1 (Ifi1) | Irgm | 15944 | 4.54 | 0.94 | Mm00492596_m1 |
lectin, galactose binding, soluble 9 | Lgals9 | 16859 | 2.53 | 0.58 | Mm00495295_m1 |
interferon gamma induced GTPase | Igtp | 16145 | 2.37 | 0.42 | Mm00497611_m1 |
lymphocyte antigen 6 complex, locus A (Ly6E.1, etc.) | Ly6a | 110454 | 2.25 | 0.39 | Mm00726565_s1 |
2'-5' oligoadenylate synthetase 1A | Oas1a | 246730 | 3.00 | NT | Mm00836412_m1 |
protein kinase, interferon inducible double stranded RNA dependent | Prkr | 19106 | 2.02 | NT | Mm00440966_m1 |
SAM domain and HD domain, 1 (Mgl1) | Samhd1 | 56045 | 1.25 | 1.62 | Mm00490121_m1 |
Type I interferon receptors | |||||
interferon (alpha and beta) receptor 1 | Ifnar1 | 15975 | 1.04 | 1.60 | Mm00439544_m1 |
interferon (alpha and beta) receptor 2 | Ifnar2 | 15976 | 0.97 | 1.44 | Mm00494916_m1 |
Type 1 interferons | |||||
MuIFN-alpha-2 interferon-alpha-2 gene | Ifna2 | 15965 | ND | NT | Mm00833961_s1 |
Interferon alpha family, gene 4 | Ifna4 | 15967 | ND | NT | Mm00833969_s1 |
Stat family | |||||
Signal transducer and activator of transcription 1(Stat1) | Stat1 | 20846 | 2.96 | NT | Mm00439518_m1 |
Signal transducer and activator of transcription 2(Stat2) | Stat2 | 20847 | 4.24 | NT | Mm00490880_m1 |