Genetic linkage of sequence-specific PCR-based molecular markers and the disease resistance gene Lanr1 of Lupinus angustifolius . Five PCR based markers, AnSeq1, AnSeq2, AnSeq3, AnSeq4 and AnSeq5, were developed in this study. The other two markers, AntjM1 and AntjM2, which were used as controls, were established previously using traditional marker development methods [12, 19]. Genetic distance in the linkage was expressed as centiMorgans. The linkage map was initially constructed using MapManager QTX  and finalized by RECORD program . These linked markers were on linkage group “NLL-11” of the lupin genetic map reported by Nelson et al.  as evidenced by the presence of the same R gene (Lanr 1) and the previous developed marker “AntjM2”.