Figure 1

Estimates of community composition for the three microbial consortia based on three different metrics. A: qPCR-based estimates of the KB-1 community from the gDNA used for generation of the three sequencing libraries, and the combined expected proportions given the amount of sequence generated from each library. qPCR estimates were based on tracking 10 dominant OTUs within the consortium[30]. B: MG-RAST-annotated prokaryotic proportions for the metagenomes’ raw reads. The MG-RAST automated annotation used was the SEED’s phylogenetic profile. All reads from the metagenomes were used to generate the initial profiles, with hits filtered by a maximum e-value of 10^-5 and a minimum alignment length of 100. C: Proportion of taxonomic assignments for the assembled metagenome datasets based on RITA taxonomic assignments. All taxonomic groups present above 1% in any of the three metagenomes were included in the proportional representations in B and C.