Transcriptional ontogeny of the developing mouse liver. A. Expression changes in fetal liver genes. Fetal liver genes were identified as detailed in the Materials and Methods. The intensity scale indicates fold-changes compared to the adult controls. Red, up-regulation; green, down-regulation; black, no change. B. RT-PCR of fetal liver gene expression in mouse livers from GD14 to PND28. *, indicates statistical significance of expression changes relative to PND28 (p ≤ 0.05); ML, maternal liver. C. In situ hybridization of Makorin 1 (Mkrn1) in the GD13.5 fetus. L, liver; Lu, lung; St, stomach; M, metanephros; SC, spinal cord. D. Global gene expression in the developing mouse liver. Genes which exhibited significant differences in expression compared to adult animals were identified as detailed in the Materials and Methods.