Canonical pathways altered in the developing liver. A. TightCluster groups genes into 4 temporal categories during liver development. Shown are examples of clusters that group genes into one of the 4 temporal categories. B. Genes expressed at different times during development fall into unique canonical pathways. Genes in the 4 temporal groups identified using TightCluster (Figure 2A) were analyzed using IPA. Only the top 10 significant pathways in the late group are shown. C. Global view of canonical pathways altered during development. Canonical pathways significantly altered at the indicated times during development compared to adult mice were identified using IPA. D. Increased expression of genes in canonical pathways involved in DNA maintenance and cell cycle (top) and cell fate signaling (bottom). E. Changes in canonical pathways of intermediary metabolism. Left, all significantly altered pathways of metabolism. Right, up-regulated pathways. For B-E, the scale numbers are the -log(p-value) and range from < 10-10 to not significant (NS). Yellow, altered pathway using all genes as input; red, up-regulated pathway using all up-regulated genes as input; green, down-regulated pathway using all down-regulated genes as input; black, not significant.