Figure 4

Inactivation of the glutamate dehydrogenase gene SHJG7666 , and measurement of intracellular glutamate (Glu) in strain 5008 and its mutant XH3 with validamycin gene cluster deleted. (A) Schematic deletion of SHJG7666 in strain 5008. (B) PCR analysis of strain 5008 and SHJG7666 mutant JG33. (C) VAL-A production in strain 5008 and mutant JG33 cultivated at 30°C or 37°C. Mean values of three independent experiments with SD are indicated by error bars. (D) Concentration of intracellular Glu in strain 5008 and mutant XH3. Samples were extracted in strain 5008 and mutant XH3 for one or two days at 30°C or 37°C. Each value is the average of two measures using an amino acid analyzer. XH3 is a mutant with val gene cluster deleted.