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Table 6 Genes significantly different in skeletal muscle of fish PP diet

From: Transcriptomic and physiological responses to fishmeal substitution with plant proteins in formulated feed in farmed Atlantic salmon (Salmo salar)

Probe name1

ACC2

FC ± SEM3

Identity4

Immune function

   

Ssa#STIR12634

TC70300

5.0 ± 1.2

Vig-2

Ssa#STIR00067_2

U66477

3.2 ± 1.3

Interferon inducible Mx protein

Ssa#CK894557

CK894557

−11.2 ± 1.4

MHC class Ib antigen

Ssa#STIR00132_2

NM_001140254

−7.4 ± 1.3

Tumor necrosis alpha-induced protein 2

Omy#NP565601

NP565601

−6.4 ± 1.4

T-cell receptor beta

Ssa#S31982089

S31982089

−5.4 ± 1.1

Platelet-activating factor receptor

Ssa#STIR12498

TC70105

−4.6 ± 1.2

γ-ip (CXCL10)

Ssa#S48440415

S48440415

−3.4 ± 1.1

SMAD3

Omy#S15331473

S15331473

−3.2 ± 1.2

Interferon-inducible protein Gig2-like

Ssa#S35544087

S35544087

−3.1 ± 1.3

B-cell CLL/lymphoma 7 protein family member B

Ssa#STIR13083

TC70912

−3.6 ± 1.0

SAMHD1

Omy#S18153399

S18153399

−2.4 ± 1.1

CD80

Ssa#KSS5035

KSS5035

−2.2 ± 1.2

Interferon regulatory factor 2-binding protein

Ssa#STIR00071_3

DW580947

−2.3 ± 1.1

Interferon -induced protein 35

Ssa#STIR08451

TC64790

−2.2 ± 1.2

Heat shock protein 47

Ssa#S35480903

S35480903

−2.0 ± 1.2

Heat shock protein 30

Ssa#STIR29454

TC95297

−2.1 ± 1.1

Beta defensin 1

Cell proliferation and apoptosis

   

Ssa#S35602638

S35602638

3.7 ± 1.4

Tripartite motif 39

Ssa#TC110067

TC110067

3.5 ± 1.5

Cell death inducing protein

Ssa#STIR08668

TC65039

3.1 ± 1.1

Bh3 interacting domain death agonist

Ssa#STIR12507

TC70118

3.0 ± 1.3

Syndecan 4

Ssa#STIR02208

BT049868

−38.6 ± 1.2

Caspase 14

Ssa#S35559333

S35559333

−4.1 ± 1.3

Ankyrin repeat domain-containing protein 54

Omy#TC151190

TC151190

−2.9 ± 1.2

Nucleostemin

Ssa#S35550715

S35550715

−2.8 ± 1.2

Growth hormone-inducible transmembrane protein

Ssa#S35585784

S35585784

−2.6 ± 1.1

Caspase-8

Ssa#KSS5035

KSS5035

−2.4 ± 1.2

Interferon regulatory factor 2-binding protein 2-B

Protein metabolism

   

Ssa#S35682089

S35682089

4.2 ± 1.3

Ubiquitin-like protein 1

Ssa#STIR17445

TC77227

2.6 ± 1.3

Acta1 protein

Ssa#STIR21465

TC83266

2.5 ± 1.2

Actin-binding

Ssa#STIR03710

BT048358

2.0 ± 1.2

Proteasome subunit beta type-9

Ssa#STIR00115_4

BT045917

2.0 ± 1.2

Tropomyosin-1 alpha chain

Ssa#S32008331

S32008331

−3.6 ± 1.7

40 S ribosomal protein S16

Ssa#CL224Ctg1

NM_001140473

−2.8 ± 1.5

Receptor-interacting serine/threonine-protein kinase 4

Omy#CX150460

CX150460

−2.7 ± 1.1

60 S ribosomal protein L27

Omy#S15320960

S15320960

−2.6 ± 1.1

Cathepsin D

Ssa#TC111443

TC111443

−2.5 ± 1.1

Serine/threonine-protein kinase 35

Ssa#FC072705

FC072705

−2.3 ± 1.0

40 S ribosomal protein S10

Ssa#S35583213

S35583213

−2.1 ± 1.2

Serine/threonine-protein kinase PINK1

Ssa#S35591236

S35591236

−2.1 ± 1.1

Histidine triad nucleotide-binding protein 3

Energy metabolism

   

Ssa#STIR39924

TC110855

2.5 ± 1.1

Cytochrome P450

Ssa#S32006874

S32006874

2.2 ± 1.1

Peroxisomal membrane protein 11 C

Ssa#KSS5038

KSS5038

−4.0 ± 1.3

Phosphoglycerate kinase

Ssa#STIR19484

TC80264

−3.1 ± 1.3

Cytochrome c oxidase polypeptide viii

Ssa#CL200Ctg1

BT059338

−2.8 ± 1.3

L-lactate dehydrogenase B chain

Ssa#S30282925

S30282925

−2.7 ± 1.1

Peroxisomal 3.2-trans-enoyl-CoA isomerase

Omy#CX150319

CX150319

−2.4 ± 1.2

Cytochrome c oxidase subunit II

Ssa#STIR13627

TC71700

−2.1 ± 1.2

Cox18 cytochrome c oxidase

Ssa#EL698167

EL698167

−2.0 ± 1.1

Malate dehydrogenase 1

  1. List of selected mRNAs found to be higher or slower in expression following the feeding trial in mid intestine (a), liver (b) or skeletal muscle (c) and grouped according to functional classes (shown in bold). The selection was based on manual assignment of function and genes with greatest fold differences in expression are presented, the genes that are lower in expression are denoted by (−) value. The genes shown were significant at p < 0.05 following t- tests and greater than 2-fold change. 1Indicates the unique code for the feature on the microarray, 2Accession number of the cDNA sequence. 3Fold-change for genes higher or lower expressed in fish fed PP relative to fish fed MP diet. 4Identity of the probe target as determined by BLASTX and BLASTN searches.