Table 7 Primers used for real time PCR for expression analysis, Acc is accession number from NCBI
Gene name | Primer name | Primer sequence(5-prime to 3-prime) | Acc | Product size | Ann T | Tissue |
|---|---|---|---|---|---|---|
Fabp2 | FatF | GCTCTGTACTAGCTCTCCTCCC | CB509140 | 156 bp | 55 °C | Mid intestine |
| Â | FatR | GGCGTACAGTTTGACTATGCAC | Â | Â | Â | Â |
Caspase-14 | Cas14F | CGATTATACACCCGGACTATGG | S35582566 | 155p | 55 °C | Mid intestine |
| Â | Cas14R | CCTATCAAGTGTGAATCCATGC | Â | Â | Â | Â |
TCR alpha | TCRaF | GGAAGACTCTGCTCTGTACCAC | U50991 | 147 bp | 55 °C | Mid intestine |
| Â | TCRaR | GCTGTGGTATTTCTTGACTTC | Â | Â | Â | Â |
IgM heavy chain | IgMF | GCTTATAGCCATAGTACTACTG | S18892409 | 169 bp | 55 °C | Mid intestine |
| Â | IgMR | GCATAGCTGCCCCATATCGC | Â | Â | Â | Â |
Tpm1 | Trop1F | CGAAGATGAGAGAGATAAAGTGC | TC76471 | 134 bp | 55 °C | Mid intestine |
| Â | Trop1R | CCTCCTCAACCAGCTGGATACG | Â | Â | Â | Â |
RFC3 | ReplF | GCTGACTCACTGCATTCCTCCTG | S34421775 | 163 bp | 55 °C | Mid intestine |
| Â | ReplR | GAAGGCCTCTAGGTGGTAAATGG | Â | Â | Â | Â |
HTRA1 | HTRA1F | GGTCATCTTCATACAGAGAGG | S35582821 | 152 bp | 55 °C | Liver |
| Â | HTRA1R | GCTTAGAGAATACCATCTTGC | Â | Â | Â | Â |
TGF beta receptor | TGBF | CCACAAGAAGCCAGCTGTCAG | gi|209735249 | 135 bp | 55 °C | Liver |
| Â | TGFBR | CTAGCCAGGTATCTCTATCATGG | Â | Â | Â | Â |
MRPS18A | 28 S F | CCATTGATTCAGTGAAGCCCATC | DW574268 | 158 bp | 55 °C | Liver |
| Â | 28 S R | CCTGCTGTGAGTTGACATGC | Â | Â | Â | Â |
Timd2 | TcellF | CCATGGACAACCACACACACTG | CA368982 | 141 bp | 55 °C | Liver |
| Â | TcellR | CCAGTAGAATGGACACCAGGATC | Â | Â | Â | Â |
Hepcidin I | HepF | GCTTCTGCTGCAAATTCTGAGG | gi|209736931 | 157 bp | 55 °C | Liver |
| Â | HepR | GTACAAGATTGAGGTTGTGCAG | Â | Â | Â | Â |
TCR alpha | TCRaF | GGAAGACTCTGCTCTGTACCAC | U50991 | 147 bp | 55 °C | Liver |
| Â | TCRaR | GCTGTGGTATTTCTTGACTTC | Â | Â | Â | Â |
CDIP | CellF | CCATGTCTGAGACCTACTCTATG | TC110067 | 243 bp | 55 °C | Skeletal muscle |
| Â | CellR | GATAGTCACTTGATGTCCAGTG | Â | Â | Â | Â |
acta1 protein | ActaF | CCTGTAAACTGTGAATGCGTC | TC77227 | 156 bp | 55 °C | Skeletal muscle |
| Â | ActaR | CCAAAGTTTTATATCAGCTGC | Â | Â | Â | Â |
TGF beta 1 | TGF1bF | GCTCGGAGTGTGAGACAGAACTG | S15319964 | 187 bp | 55 °C | Skeletal muscle |
| Â | TGF1bR | CACTTGACGCAACAGAAACACTCC | Â | Â | Â | Â |
RT1-CE5 | MHC1bF | GGAAAGATCTCCTGAAGACTTGAG | CK894557 | 101 bp | 55 °C | Skeletal muscle |
| Â | MHC1bR | CGTTTATGAGAAGTTCAGC | Â | Â | Â | Â |
60 S rib prot | 60SF | GCTTCTTACCATGGTTCTTCAG | DR695852 | 140 bp | 55 °C | Skeletal muscle |
| Â | 60SR | GGTCAAGATCCCATCCACCATC | Â | Â | Â | Â |
Heat shock protein 30 | HeatF | CCATCCAACCAGTCTCCTACAAGC | EG804126 | 303 bp | 55 °C | Skeletal muscle |
| Â | HeatR | CCTCCTCAGCAGATAATGGATTC | Â | Â | Â | Â |
Hprt | HprtF | CCGCCTCAAGAGCTACTGTAAT | EG866745 | 255 bp | 55 °C | All tissues |
| Â | HprtR | GTCTGGAACCTCAAACCCTATG | Â | Â | Â | Â |